Anti-NLGN4Y Polyclonal Antibody for WB, IHC-P, ELISA - Q8NFZ3

Core Product Specifications and Parameters

Biological Background: NLGN4Y Function and Localization

• Neuroligin-4, Y-linked (NLGN4Y) is encoded by the NLGN4Y gene, spanning 816 amino acids with a calculated molecular weight of 92 kDa.
• It functions as a cell surface protein that mediates cell-cell interactions through binding to neurexin family members, playing a role in synapse formation and adhesion.
• NLGN4Y is localized at the cell membrane, postsynaptic density membrane, and is enriched in dendritic spines and dendrites at synapses.
• Tissue expression is detected in fetal and adult brain, prostate, and testis, suggesting roles in neural development and reproductive tissues.
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, and contains disulfide bonds, consistent with its transmembrane and cell adhesion functions.
• Alternative splicing generates multiple isoforms, and the protein is classified under cell adhesion molecules with a signal peptide and a single transmembrane helix.
• NLGN4Y is located on the Y chromosome and is expressed exclusively in males, contributing to sex-specific neurological functions.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 92 kDa is expected; optimizing primary antibody dilution and detection conditions is recommended.
• In immunohistochemistry (paraffin sections), consider antigen retrieval methods and validate staining in human brain or testis tissue, where NLGN4Y is expressed.
• For ELISA applications, the recombinant immunogen (aa 122-256) may be used as a positive control to assess antibody binding.
• When using in mouse or rat models, confirm target sequence homology with the immunogen region to ensure cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers investigating synaptic biology and sex-linked neurologic differences, CamelBio provides one-stop procurement support from concept to clinic, covering key targets like neuroligins and neurexins.