Anti-NKX6-1 Polyclonal Antibody for WB, IHC-P, ELISA - P78426

Core Product Specifications and Parameters

Biological Background: NKX6-1 Function and Localization

• Homeobox protein Nkx-6.1, also known as NKX6A or NKX6.1, is a transcription factor belonging to the class II neuronal progenitor factors induced by SHH signals.
• It binds to specific A/T-rich DNA sequences in promoter regions, acting as both an activator and repressor of gene expression.
• Essential for the development of insulin-producing beta cells in the islets of Langerhans during the secondary transition.
• Together with NKX2-2 and IRX3, restricts the generation of motor neurons to the appropriate region of the neural tube.
• Localizes exclusively to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Expressed in pancreatic beta cells, highlighting its tissue-specific function in endocrine pancreas development.
• Classified under keywords including Activator, Developmental protein, DNA-binding, Homeobox, Methylation, Nucleus, Repressor, and Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1-100) of human NKX6-1; consider this epitope location when designing detection strategies.
• Validated applications include Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA; optimize dilution and conditions experimentally for each assay.
• Cross-reactivity is reported for human, mouse, and rat; verify performance in the target species and tissue type, especially given the expression in pancreatic beta cells.
• The predicted molecular weight is ~38 kDa; use appropriate molecular weight markers and confirm band specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This NKX6-1 polyclonal antibody supports research in diabetes, developmental biology, and neuroscience, and we can supply complementary reagents to accelerate your diagnostic or research workflows.