Anti-NKX3.1 Rabbit Monoclonal Antibody for WB and ELISA - Q99801

Core Product Specifications and Parameters

Biological Background: NKX3.1 Function and Localization

• NKX3.1 is a homeobox transcription factor, also known as Homeobox protein Nkx-3.1 or NK-3 homolog A, encoded by the human NKX3-1 gene.
• It binds preferentially to the consensus sequence 5'-TAAGT[AG]-3' and functions as a transcriptional repressor, regulating gene expression through its DNA-binding homeodomain.
• Plays a critical role in normal prostate development by controlling proliferation of glandular epithelium and duct formation.
• Acts as a tumor suppressor in prostate carcinogenesis, inhibiting proliferation and invasion of PC-3 prostate cancer cells.
• Localizes to the nucleus, consistent with its role as a DNA-binding transcriptional regulator.
• Highly expressed in prostate tissue, with lower expression detected in testis.
• Related keywords include DNA-binding, homeobox, nucleus, repressor, transcription regulation, tumor suppressor, and Ubl conjugation, reflecting its functional domains and regulatory modifications.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human NKX3.1 protein (amino acids 1-234), making the antibody suitable for detecting the target in both denaturing and reducing Western blot conditions. Expected band size: ~26 kDa.
• For Western blot, validate the antibody in prostate-derived cell lines (e.g., LNCaP, PC-3) or tissue lysates to confirm specificity.
• For ELISA, consider optimizing coating antigen and detection conditions based on the protein's native conformation or recombinant fragments.
• Since cross-reactivity is confirmed for human, ensure sample species compatibility before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing. This anti-NKX3.1 monoclonal antibody is part of our portfolio targeting prostate cancer biomarkers, and we can supply validated antibody pairs, bulk ancillary reagents, or assist in rare target raw-material procurement to streamline your assay development from concept to clinic.