Anti-NKX3.1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99801

Core Product Specifications and Parameters

Biological Background: NKX3.1 Function and Localization

• Homeobox protein Nkx-3.1, also known as NKX3.1 or NKX3A, is a transcription factor that binds preferentially to the consensus sequence 5'-TAAGT[AG]-3' and can function as a transcriptional repressor.
• NKX3.1 plays a critical role in normal prostate development, regulating proliferation of glandular epithelium and ductal morphogenesis.
• Acts as a tumor suppressor in prostate carcinogenesis, with the ability to inhibit proliferation and invasion of prostate cancer cells (e.g., PC-3 line).
• Localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Exhibits high expression in the prostate and lower expression in the testis, highlighting its tissue-specific regulatory functions.
• The protein is subject to alternative splicing, producing multiple isoforms, and is regulated by ubiquitination (Ubl conjugation).
• Belongs to the homeobox family, containing a characteristic DNA-binding homeodomain; also listed under keywords such as 3D-structure, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1-80 of human NKX3.1, which is the N-terminal region. This region may detect multiple isoforms. Verify specificity by knockdown or knockout controls.
• For Western blot, expect a band at approximately 26 kDa. Optimize loading and transfer conditions for this relatively low molecular weight protein.
• For immunofluorescence/ICC, confirm nuclear localization. Use appropriate fixation and permeabilization protocols for nuclear antigens.
• When working with human and mouse samples, validate the antibody's reactivity in the relevant tissue (e.g., prostate or testis) due to potential species-specific epitope recognition.
• For ELISA applications, consider using the recombinant immunogen as a positive control to establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. As an NKX3.1 polyclonal antibody, this product facilitates studies in prostate cancer biology and tumor suppression. Our comprehensive portfolio and technical consulting streamline the development of IVD raw materials from concept to clinic.