Anti-NKX3.1 Monoclonal Antibody for WB, IHC-P, ELISA - Q99801

Core Product Specifications and Parameters

Biological Background: NKX3.1 Function and Localization

• Protein identity: Homeobox protein Nkx-3.1 (NKX3-1, also designated Homeobox protein NK-3 homolog A) is a transcription factor encoded by the NKX3-1 gene and characterized by a homeobox DNA-binding domain. Alternative splicing produces multiple isoforms.
• DNA-binding and transcription regulation: NKX3.1 preferentially binds the consensus sequence 5'-TAAGT[AG]-3' and functions primarily as a transcriptional repressor, modulating gene expression programs in target cells.
• Prostate development: It plays a critical role in normal prostate development, controlling proliferation of the glandular epithelium and directing proper ductal morphogenesis.
• Tumor suppressor: NKX3.1 acts as a tumor suppressor in prostate carcinogenesis; it has been shown to inhibit proliferation and invasion of PC-3 prostate cancer cells.
• Subcellular localization: The protein is predominantly localized to the nucleus, consistent with its role in transcriptional regulation.
• Tissue specificity: NKX3.1 is highly expressed in the prostate, with lower levels detectable in the testis.
• Post-translational modifications and molecular features: The protein is subject to ubiquitin conjugation (Ubl conjugation) and has been identified in proteomics screens. It contains a homeobox domain essential for DNA binding and transcriptional activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human NKX3.1; consider this when analyzing isoforms or truncated forms.
• For Western blot (WB), a band around 26 kDa is expected. Verify specificity using lysates from prostate or prostate cancer cell lines (e.g., LNCaP, PC-3) as positive controls, and include negative controls from tissues with low or absent expression.
• For immunohistochemistry (IHC-P), nuclear staining should be observed. Antigen retrieval and antibody dilution may require optimization depending on the tissue fixation and processing conditions.
• Due to reactivity across human, mouse, and rat, the antibody is suitable for cross-species studies, but validation in each species’ sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with integrated access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. With this anti-NKX3.1 antibody, researchers focused on prostate biology and cancer can streamline assay development from concept to clinic. Our one-stop model ensures consistent quality and supply across your entire IVD development pipeline.