Anti-NKX3-2 Rabbit Polyclonal Antibody for WB, ELISA - P78367

Core Product Specifications and Parameters

Biological Background: NKX3-2 Function and Localization

• Homeobox protein Nkx-3.2, also known as Bagpipe homeobox protein homolog 1 or homeobox protein NK-3 homolog B, is a transcription factor encoded by the NKX3-2 gene.
• Acts as a transcriptional repressor that negatively regulates chondrocyte maturation, playing a critical role in skeletal development.
• Required for distal stomach development, specifically for proper antral-pyloric morphogenesis and the formation of antral-type epithelium.
• In concert with GSC, defines structural components of the middle ear; essential for tympanic ring and gonium development, and for regulating the width of the malleus.
• Localizes to the nucleus, consistent with its function as a DNA-binding transcription regulator.
• Expressed at highest levels in cartilage, bone (osteosarcoma), and gut (small intestine and colon); moderate expression in trachea and brain; found in visceral mesoderm and embryonic skeleton.
• Keywords include DNA-binding, dwarfism, homeobox, nucleus, repressor, and transcription regulation, underscoring its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-180 of human NKX3-2, encompassing the N-terminal region including potential regulatory domains. Consider this when interpreting isoform-specific detection.
• Recommended applications are western blot (WB) and ELISA. For WB, a band around 35 kDa is expected; validate in cell or tissue lysates known to express NKX3-2 (e.g., osteosarcoma, cartilage, or gut samples).
• The antibody cross-reacts with human, mouse, and rat NKX3-2 based on supplier data. When using models from these species, perform initial validation to confirm reactivity and specificity in your experimental system.
• For ELISA, coat with the immunogen or target protein under appropriate conditions. Titrate the antibody and validate assay sensitivity using positive controls such as recombinant NKX3-2 protein or lysates from expressing tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, catering to diagnostic developers and research laboratories. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your workflows. Whether you require rare-target raw materials or robust supply chains for translational research, CamelBio bridges the gap from concept to clinic, with the NKX3-2 antibody being one example of our commitment to enabling developmental biology and skeletal disease research.