Anti-NKX2-5 Rabbit Polyclonal Antibody for WB, ELISA - P52952

Core Product Specifications and Parameters

Biological Background: NKX2-5 Function and Localization

• NKX2-5 is a cardiac-specific homeobox transcription factor essential for heart development.
• It acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4.
• It may cooperate with TBX2 to negatively modulate NPPA/ANF expression.
• It binds to the core DNA motif of the NPPA promoter. Related references: PMID:22849347 PMID:26926761
• Together with PBX1, it is required for spleen development through CDKN2B repression. Related references: PMID:22560297
• Positively regulates transcription of COL3A1 and MMP2, contributing to hypoxia-induced pulmonary endothelial fibrosis. Related references: PMID:29899023
• Subcellular localization: Nucleus.
• Tissue specificity: Expressed only in the heart.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-135) of human NKX2-5. Consider verifying the detected band size in Western blot, as the predicted molecular weight is approximately 35 kDa.
• For WB, use known positive controls from cardiac tissue lysates (human, mouse, or rat) to confirm specificity.
• For ELISA, optimize coating antigen and antibody dilution using a checkerboard titration to achieve the best signal-to-noise ratio.
• Cross-reactivity with mouse and rat has been reported; however, it is advisable to validate in the specific sample system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-NKX2-5 product, and bulk ancillary reagents. With a focus on cardiac development and congenital disease markers, we also source rare target raw materials to support your assay development from concept to clinic. Let CamelBio be your reliable partner for streamlined procurement of high-quality IVD reagents.