Anti-NKX2-2 Polyclonal Antibody for WB and ELISA - O95096

Core Product Specifications and Parameters

Biological Background: NKX2-2 Function and Localization

• NKX2-2 encodes homeobox protein Nkx-2.2, also known as homeobox protein NK-2 homolog B (NKX2B), a transcription factor containing a homeobox DNA-binding domain.
• It functions as a transcriptional activator critical for the development of insulin-producing beta cells in the endocrine pancreas.
• The protein is also implicated in specifying boundaries within the developing diencephalon and controlling genes involved in axonal guidance.
• NKX2-2 binds to regulatory elements in the NEUROD1 promoter, influencing neuronal differentiation.
• Subcellular localization is primarily nuclear, consistent with its role as a transcription factor.
• Key associated keywords include Activator, Developmental protein, DNA-binding, Homeobox, Nucleus, Reference proteome, and Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 102-201 of human NKX2-2 (NP_002500.1). Consider using Western blot to detect endogenous protein around 30 kDa in human cell lines or tissues known to express NKX2-2 (e.g., pancreatic beta cells or neuronal tissues).
• For ELISA, the antibody may be suitable as a capture or detection reagent; typical optimization with antigen coating and antibody titration is advised.
• Since the antibody is polyclonal, it may recognize multiple epitopes, potentially providing robust signal in denaturing and native conditions. Validate specificity in the relevant experimental system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including this anti-NKX2-2 polyclonal antibody suitable for pancreatic development and neuroscience research. Our supply capabilities span validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. Contact us to streamline your procurement from concept to clinic.