Anti-NKX2-1 Rabbit Polyclonal Antibody for WB, ELISA - P43699

Core Product Specifications and Parameters

Biological Background: NKX2-1 Function and Localization

• Homeobox protein Nkx-2.1 (also known as TTF-1, T/EBP, NKX2A) is a transcription factor encoded by the NKX2-1 gene on human chromosome 14.
• Binds and activates thyroid-specific gene promoters (e.g., thyroglobulin, thyroperoxidase, thyrotropin receptor), critical for thyroid differentiation.
• Plays a key role in lung development and surfactant homeostasis; forms a regulatory loop with GRHL2 to coordinate lung epithelial cell morphogenesis.
• Activates GNRHR transcription and enhances circadian gene expression oscillation; represses NR1D1, linking to biological rhythms.
• Located predominantly in the nucleus, consistent with its DNA-binding homeobox domain function.
• Tissue-specific expression in thyroid and lung, with disease variants associated with congenital hypothyroidism.
• Post-translational modifications include phosphorylation; classified as a phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–110 (N-terminal region) of human NKX2-1; verify that it matches the target isoform(s) in your experimental system.
• For Western blot (WB), optimize primary antibody dilution (e.g., 1:500–1:2000) and use appropriate molecular weight markers (predicted ~39 kDa).
• For ELISA, consider using this polyclonal antibody as a capture or detection reagent after confirming specificity with purified antigen or cell lysates.
• Cross-reactivity is reported for human and rat; additional species may require validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibodies like anti-NKX2-1 for thyroid and lung transcription factor studies. We offer monoclonal and polyclonal antibody pairs, bulk ancillary reagents, and support for rare target sourcing, streamlining your path from concept to clinical application.