Anti-NKRF Polyclonal Antibody for WB, ELISA - O15226

Core Product Specifications and Parameters

Biological Background: NKRF Function and Localization

• Transcriptional repressor: binds the negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to silence NF-kappa-B-responsive genes (PubMed:10562553). Related references: PMID:10562553
• Functions as a cofactor for DHX15, enhancing its ATPase activity by stabilizing a functional conformation with high RNA affinity (PubMed:12381793). Related references: PMID:12381793
• Constitutively silences the interferon beta promoter and inhibits basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Related references: PMID:12381793
• Involved in the regulation of IL-8 transcription (PubMed:12381793). Related references: PMID:12381793
• Subcellular localization predominantly in the nucleolus (nucleus).
• Ubiquitously expressed with lower levels in colon, peripheral blood lymphocytes, lung, and kidney (PubMed:10562553). Related references: PMID:10562553
• Undergoes post-translational modifications including phosphorylation and ubiquitination.
• Alternatively spliced isoforms exist, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band at approximately 78 kDa. Consider using protein extracts from tissues with known NKRF expression, such as HeLa or HEK293 cells, as positive controls.
• In ELISA, optimize the coating antigen concentration and antibody dilution using a checkerboard titration. Cross-reactivity with mouse and rat orthologs suggests potential use in these species, but validation in the relevant sample system is recommended.
• The immunogen spans amino acids 391–690, a region likely to be exposed in native and denatured conditions, supporting both WB and ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement. This NKRF antibody supports research into transcriptional regulation, immune response pathways, and inflammatory disease mechanisms. Leverage CamelBio’s expertise to accelerate your diagnostic development from concept to clinic.