Anti-NKD1 Rabbit Polyclonal Antibody for WB, ELISA - Q969G9

Core Product Specifications and Parameters

Biological Background: NKD1 Function and Localization

• NKD1 (Protein naked cuticle homolog 1) is encoded by the NKD1 gene, functioning as a cell-autonomous antagonist of the canonical Wnt signaling pathway.
• It may also activate a non-canonical Wnt pathway that regulates planar cell polarity, highlighting its dual role in Wnt signaling modulation.
• The protein localizes to the cell membrane and cytoplasm, consistent with its membrane-associated functions.
• NKD1 is broadly expressed, with detected levels in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, and spleen.
• Key structural features include myristoylation, calcium-binding capacity, and metal-binding sites, classifying it as a lipoprotein.
• The canonical molecular weight is approximately 52.3 kDa (UniProt: 52285 Da), closely matching the observed 52 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human NKD1, suggesting the antibody targets the N-terminal region; consider sequence alignment to assess isoform specificity.
• For WB, a band around 52 kDa is expected in human lysates; pre-adsorption with the immunogen peptide may confirm specificity.
• ELISA performance can be optimized by titrating the antibody against the peptide or recombinant NKD1 protein; cross-reactivity with other species should be validated if used beyond human samples.
• Given the ubiquitous expression, loading controls such as GAPDH or β-actin are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material sourcing, supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This NKD1 antibody is particularly suited for Wnt signaling pathway research, and we can assist in sourcing rare target raw materials to accelerate your assay development from concept to clinic.