Anti-NIPSNAP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BPW8

Core Product Specifications and Parameters

Biological Background: NIPSNAP1 Function and Localization

• NIPSNAP1 (Protein NipSnap homolog 1) functions as a mitophagy receptor that promotes the clearance of damaged mitochondria by recruiting the autophagy machinery.
  Related references: PMID:30982665

• Upon mitochondrial depolarization, NIPSNAP1 accumulates on the outer mitochondrial membrane, serving as an 'eat me' signal for autophagic degradation.
  Related references: PMID:30982665

• NIPSNAP1 directly interacts with key autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1, WDFY3/ALFY) and ATG8 family proteins (LC3A/B/C, GABARAP, GABARAPL1/2) to mediate selective autophagy.
  Related references: PMID:30982665

• The protein is predominantly localized to the mitochondrial matrix under basal conditions.

• Post‑translational modifications including acetylation, phosphorylation, and ubiquitin‑like conjugation have been identified, which may regulate its function.

• Ubiquitous expression with highest levels observed in the liver.
  Related references: PMID:9661659

Experimental Guidance and Technical Tips

• The immunogen targets residues 70–145 of human NIPSNAP1; consider epitope accessibility in native versus denaturing conditions.
• For Western blot, the predicted molecular weight is ~33 kDa; validate band specificity using knockout or siRNA‑treated controls in relevant sample systems.
• In ELISA applications, optimize coating antigen concentration and antibody dilution to achieve a robust signal‑to‑noise ratio.
• Cross‑reactivity with mouse and rat orthologs is documented; however, confirm detection in your specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research labs, providing reliable access to anti‑NIPSNAP1 antibodies and related mitochondrial research reagents. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and rare‑target custom sourcing—all supported by technical consulting to accelerate your project from discovery to clinical application.