Anti-NIP7 Polyclonal Antibody for WB and ELISA - Q9Y221

Core Product Specifications and Parameters

Biological Background: NIP7 Function and Localization

• NIP7, also designated as 60S ribosome subunit biogenesis protein NIP7 homolog, is encoded by the NIP7 gene (aliases: KD93, CGI-37, HSPC031).
• It plays a critical role in ribosome biogenesis, specifically required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
• The protein localizes to the nucleolus within the nucleus, consistent with its function in ribosomal RNA maturation.
• NIP7 is expressed in hematopoietic stem/progenitor cells, suggesting involvement in early blood cell development.
• It possesses RNA-binding activity and is subject to alternative splicing, which may generate multiple isoforms.
• UniProt keywords include 3D-structure, proteomics identification, and reference proteome, indicating a well-characterized protein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-180 of human NIP7, targeting the full-length region. In Western blot, expect a band around 20 kDa.
• Due to the polyclonal nature, the antibody may recognize multiple isoforms or post-translationally modified variants; validate in your specific sample system.
• For human samples, consider using HeLa or HEK293 cell lysates; for mouse samples, NIH/3T3 or relevant tissue extracts.
• Perform a standard WB protocol with optimization of antibody dilution; starting range 1:500–1:2000 is commonly used for polyclonal antibodies.
• For ELISA, coat antigen at 1–10 µg/mL and titrate antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B sourcing partner for IVD raw materials, offering this NIP7 polyclonal antibody along with a broad portfolio of validated antibodies and bulk ancillary reagents. Whether you're developing assays for ribosome biogenesis research or hematopoietic studies, we provide one-stop access to rare-target antibodies and customizable procurement services. Contact us to streamline your supply chain and accelerate your project from concept to clinic.