Anti-NINL Rabbit Polyclonal Antibody for WB and ELISA - Q9Y2I6

Core Product Specifications and Parameters

Biological Background: NINL Function and Localization

• NINL (Ninein-like protein), also designated as NLP, is encoded by the gene NINL (KIAA0980) in humans, and is implicated in microtubule organization and centrosome function.
• In interphase cells, NINL participates in microtubule organization; its overexpression causes Golgi fragmentation, peripheral lysosome dispersion, and interferes with mitotic spindle assembly.
• The protein is also involved in vesicle transport within photoreceptor cells (by similarity) and may contribute to ovarian carcinogenesis.
• NINL localizes primarily to the centrosome, a major microtubule-organizing center, and is distributed throughout the cytoplasm.
• Expression patterns are cell-cycle-dependent, with protein levels peaking during the G2/M phase, and are observed in specific carcinoma cell lines (e.g., KYSE-150, HeLa, U2OS) as well as fetal and adult tissues including heart, skeletal muscle, liver, lung, cochlea, brain, testis, kidney, and retina.
• Key structural and regulatory features include alternative splicing, calcium- and metal-binding domains, coiled-coil motifs, and post-translational modifications such as phosphorylation and ubiquitination, reflecting its diverse cellular roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–190) of human NINL, suggesting that the antibody may recognize epitopes within this domain; consider verifying reactivity with known NINL isoforms produced by alternative splicing.
• For Western blotting, validate antibody specificity using human and mouse cell lysates given the reported cross-reactivity; a band near the predicted molecular weight of approximately 156 kDa should be expected.
• In ELISA, use recombinant NINL protein as a standard for coating optimization and to assess antibody affinity; include appropriate negative controls to confirm specificity.
• Due to cell-cycle-dependent expression, consider synchronizing cell populations or collecting samples at the G2/M phase if improved detection is needed.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we specialize in providing comprehensive IVD raw material solutions, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows. This anti-NINL polyclonal antibody is part of our extensive portfolio, offering a reliable tool for studying microtubule dynamics, centrosome biology, and cancer-related pathways. With our integrated sourcing model and technical consulting, we help bridge the gap from concept to clinic, ensuring you have access to the rare and critical raw materials needed for your projects.