Anti-Ninein (NIN/SCKL7) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N4C6

Core Product Specifications and Parameters

Biological Background: Ninein Function and Localization

• Ninein is a centrosomal protein encoded by the NIN gene, also known as glycogen synthase kinase 3 beta-interacting protein (GSK3B-interacting protein).
• Ninein is required for positioning and anchorage of the microtubule minus-end in epithelial cells. Related references: PMID:15190203 PMID:23386061
• It acts as a centrosome maturation factor. Related references: PMID:11956314
• Ninein plays a role in microtubule nucleation by recruiting the gamma-tubulin ring complex to the centrosome. Related references: PMID:15190203
• Overexpression of ninein suppresses release of microtubules without perturbing nucleation or elongation. Related references: PMID:15190203
• Required for centriole organization and microtubule anchoring at the mother centriole. Related references: PMID:23386061
• Subcellular localization includes cytoplasm, centrosome, centriole, cell junctions, and desmosomes.
• Ubiquitously expressed with highest levels in heart and skeletal muscle. Isoform 1 is more abundant than isoform 5.
• Contains coiled-coil domains, GTP-binding motifs, and is subject to alternative splicing and phosphorylation. Associated with disease variants (dwarfism, intellectual disability).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-287), so this antibody may recognize multiple isoforms sharing this region. Consider validating with isoform-specific controls.
• For Western blotting, the target protein weight is ~243 kDa. Use low-percentage gels and extended transfer times to ensure efficient detection.
• In IHC-P, perform antigen retrieval and optimize blocking steps. Positive control tissues with high ninein expression (e.g., heart, skeletal muscle) are recommended.
• Cross-reactivity with mouse and rat suggests epitope conservation. Validate reactivity in these species with appropriate positive and negative controls.
• For ELISA, determine optimal coating concentration and antibody dilution experimentally to achieve desired sensitivity and specificity.

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CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing services support diagnostic manufacturers and researchers in reliably obtaining rare target antibodies like Ninein (NIN) for centrosome and microtubule research. From concept to clinic, CamelBio streamlines procurement to accelerate your assay development.