Anti-Niemann Pick C1 Monoclonal Antibody for WB - O15118

Core Product Specifications and Parameters

Biological Background: Niemann Pick C1 Function and Localization

• NPC1 is a 142 kDa multi‑pass transmembrane glycoprotein and the product of the NPC1 gene (UniProt O15118).
• It functions as an intracellular cholesterol transporter, acting together with NPC2 to mediate cholesterol egress from late endosomes/lysosomes.
  Related references:
  PMID:10821832, PMID:12554680, PMID:18772377
• Unesterified cholesterol released from LDLs is transferred by NPC2 to the N‑terminal cholesterol‑binding pocket of NPC1, where the hydroxyl group is buried in the pocket.
  Related references:
  PMID:18772377, PMID:19563754, PMID:27238017
• NPC1 tethers endosomal/lysosomal compartments to endoplasmic reticulum contact sites via interaction with GRAMD1B, enabling cholesterol transfer to the ER.
  Related references:
  PMID:31537798
• The protein binds oxysterol with higher affinity than cholesterol and may contribute to vesicular trafficking in glial cells, supporting nerve terminal integrity.
• NPC1 inhibits cholesterol‑mediated mTORC1 activation by interacting with SLC38A9.
  Related references:
  PMID:28336668
• As an endosomal entry receptor for ebolavirus, NPC1 is implicated in host–virus interactions.
• Mutations in NPC1 lead to Niemann‑Pick disease type C, a lysosomal storage disorder characterized by cholesterol accumulation.
• The protein localizes predominantly to the limiting membrane of late endosomes and lysosomes, consistent with its role in intracellular cholesterol distribution.

Experimental Guidance and Technical Tips

• The immunogen was designed from the C-terminal region (amino acids 1179‑1278) of human NPC1; this region may exhibit limited cross‑species conservation—validate in the intended species with appropriate controls.
• For Western blot, the predicted molecular weight is 142 kDa; use a low‑percentage polyacrylamide gel and ensure efficient protein transfer, as large transmembrane proteins may require extended transfer times.
• For IHC‑P and IF/ICC, test different antigen retrieval protocols and fixatives to optimize detection; include isotype controls to confirm signal specificity.
• The antibody cross‑reacts with mouse and rat NPC1, but always verify cross‑reactivity using positive and negative lysates or tissue sections from your target species.
• When used in ELISA, titrate the antibody to determine the optimal working concentration for your antigen‑coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop sourcing bridge for high‑quality IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents that accelerate diagnostic development. This anti‑NPC1 monoclonal antibody exemplifies our capability to supply optimized, monospecific reagents for cholesterol metabolism and lysosomal storage disease research. We also provide custom antibody development, bulk production, and sourcing of rare targets to support your entire product lifecycle.