Anti-NID1 Rabbit Polyclonal Antibody for WB, ELISA - P14543

Core Product Specifications and Parameters

Biological Background: Nidogen-1 Function and Localization

• Nidogen-1, also known as entactin, is a sulfated glycoprotein and a major component of basement membranes.
• It mediates cell-extracellular matrix interactions by binding to laminin, collagen IV, and perlecan.
• The protein contains EGF-like domains, disulfide bonds, and calcium-binding regions, and undergoes alternative splicing.
• Nidogen-1 is secreted and localizes to the extracellular matrix, specifically in the basement membrane.
• It plays a critical role in cell adhesion and tissue organization.
• The NID1 gene encodes a reference proteome protein with a molecular weight of approximately 136 kDa.
• Post-translational modifications include sulfation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 110-400) of human nidogen-1, which may recognize both full-length and potential isoforms.
• For Western blot (WB), a band around 136 kDa is expected; consider using reducing conditions and appropriate gel percentage.
• For ELISA, the antibody can be used to detect nidogen-1 in human serum, plasma, or cell culture supernatants; validate in the relevant sample system.
• Since the antibody is polyclonal, it may offer robust detection but lot-to-lot variability should be monitored.
• Cross-reactivity with other species has not been reported; verify if needed for non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, from concept to clinic. This nidogen-1 antibody supports basement membrane and extracellular matrix research, and we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to streamline your diagnostic development.