Anti-NHP2 Polyclonal Antibody for WB, ELISA - Q9NX24

Core Product Specifications and Parameters

Biological Background: NHP2 Function and Localization

• H/ACA ribonucleoprotein complex subunit 2 (NHP2), also known as nucleolar protein family A member 2 (NOLA2) or snoRNP protein NHP2, is a core component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex.
• NHP2 is essential for ribosome biogenesis and telomere maintenance. Within the H/ACA snoRNP, it catalyzes the pseudouridylation of rRNA, converting uridine to pseudouridine by isomerizing the ribose linkage from N1 to C5. Each rRNA may contain up to 100 pseudouridine residues, which contribute to stabilizing rRNA conformation.
• The protein is also implicated in the correct processing and intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme, thereby influencing telomere homeostasis.
• Subcellularly, NHP2 localizes to the nucleus, where it is enriched in nucleoli and Cajal bodies, consistent with its roles in rRNA modification and telomerase RNA maturation.
• NHP2 is broadly expressed across human tissues, including brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis, and thymus, with relatively lower levels in the liver.
• Post-translational modifications include phosphorylation and isopeptide bond formation (Ubl conjugation). Mutations in NHP2 are associated with Dyskeratosis congenita, a telomere biology disorder characterized by bone marrow failure, skin abnormalities, and cancer predisposition.

Experimental Guidance and Technical Tips

• The polyclonal antibody was generated against a recombinant protein spanning amino acids 1–153 of human NHP2; it is expected to recognize full-length NHP2. Cross-reactivity with rat NHP2 is reported; consider verifying reactivity in other species of interest.
• For Western blotting, the predicted molecular weight of NHP2 is approximately 17 kDa. Use appropriate gel compositions (e.g., 12–15% SDS‑PAGE) and transfer conditions for low‑molecular‑weight proteins.
• In ELISA applications, optimal coating antigen concentrations and detection antibody dilutions should be determined empirically. Consider using recombinant NHP2 or cell lysates as positive controls.
• As a nuclear/nucleolar protein, sample preparation for WB should include effective nuclear enrichment or use of appropriate lysis buffers to solubilize NHP2.
• No quantitative performance data is provided; always validate the antibody in your specific experimental system with suitable controls.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one‑stop platform for IVD raw materials, technical services, and consulting, supporting projects from concept to clinic.
• This anti‑NHP2 polyclonal antibody is part of CamelBio’s portfolio for ribosomal biogenesis and telomere research, complementing our supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing.
• Whether you require reliable antibodies for assay development or customized procurement solutions, CamelBio bridges the gap between your diagnostic goals and high‑quality raw materials.