Anti-NHLRC1 Polyclonal Antibody for WB, IHC-P, ELISA - Q6VVB1

Core Product Specifications and Parameters

Biological Background: NHLRC1 Function and Localization

• NHLRC1 encodes the E3 ubiquitin-protein ligase malin, an NHL repeat-containing RING-type zinc-finger protein that functions as a ubiquitin transferase.
• Together with the phosphatase EPM2A/laforin, malin mediates the clearance of toxic polyglucosan and misfolded protein aggregates through both the ubiquitin-proteasome system (UPS) and macroautophagy.
• In a laforin-dependent manner, malin ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D, targeting them for proteasomal degradation, thereby reducing glycogen accumulation.
• Malin polyubiquitinates its partner EPM2A/laforin and the glycogen debranching enzyme AGL, promoting their proteasome-dependent turnover.
• Subcellular localization studies place malin at the endoplasmic reticulum and in the nucleus, consistent with roles in protein quality control and stress responses.
• Tissue expression is broad, with notable levels in brain, cerebellum, spinal cord, heart, liver, skeletal muscle, and pancreas.
• Defects in NHLRC1 are associated with Lafora disease, a progressive myoclonus epilepsy with neurodegeneration, underscoring the protein’s critical role in neuronal glycogen homeostasis.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1–280 amino acids of human NHLRC1; therefore, the antibody may detect full-length malin (~42 kDa) and any endogenous N-terminal fragments in denaturing Western blot.
• For WB, consider a standard PVDF membrane and pre-block with 5% non-fat milk to reduce background; validate the band pattern in relevant tissue lysates such as brain or muscle.
• In IHC-P, given the protein’s abundance in the central nervous system, brain or spinal cord sections are recommended for initial testing; heat-mediated antigen retrieval (pH 6 citrate buffer) may improve nuclear/ER epitope accessibility.
• Since this is a polyclonal antibody, lot-to-lot consistency should be confirmed by the end user in their specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research labs, and clinical groups with validated IVD raw materials, including this NHLRC1 polyclonal antibody for neurodegeneration and glycogen metabolism studies. As a dedicated B2B partner, we offer optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, all supported by expert technical consulting from concept to clinic.