Anti-NHLH1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q02575

Core Product Specifications and Parameters

Biological Background: NHLH1 Function and Localization

• NHLH1 (Helix-loop-helix protein 1), also known as NSCL-1, HEN1, or bHLHa35, is a class A basic helix-loop-helix (bHLH) transcription factor encoded by the NHLH1 gene in humans.
• The protein contains a conserved bHLH domain that mediates DNA binding and dimerization, allowing it to regulate transcription of target genes.
• NHLH1 is thought to function as a DNA-binding protein involved in the control of cell-type determination, particularly within the developing nervous system.
• It is classified as a developmental protein and differentiation factor, suggesting a role in tissue-specific gene expression programs.
• Subcellularly, NHLH1 localizes to the nucleus, consistent with its role in transcriptional regulation.
• UniProt keywords annotate NHLH1 as a DNA-binding transcription factor involved in developmental processes.

Experimental Guidance and Technical Tips

• As the immunogen corresponds to the N-terminal region (amino acids 1-60), the antibody may recognize the full-length protein and possibly N-terminal fragments; confirm specificity in your system.
• For Western blot (WB), consider loading appropriate human protein lysates with a predicted band size around 15 kDa; validate with positive and negative controls.
• In immunofluorescence (IF/ICC), nuclear localization should be expected; optimize fixation and permeabilization to preserve epitope accessibility.
• For immunoprecipitation (IP), titrate antibody amount and use a suitable secondary antibody-conjugated resin; crosslinking may improve capture efficiency.
• ELISA applications may use this antibody as a capture or detection reagent; perform titration to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical services, supporting every stage from concept to clinic. This anti-NHLH1 polyclonal antibody is part of our extensive portfolio of transcription factor and neurobiology reagents, ideal for developmental research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development and manufacturing needs.