Anti-NHLH1 Monoclonal Antibody for WB, IP, ELISA, ChIP - Q02575

Core Product Specifications and Parameters

Biological Background: NHLH1 Function and Localization

• NHLH1 (also known as HEN1, NSCL, bHLHa35) is a class A basic helix-loop-helix (bHLH) transcription factor encoded by the NHLH1 gene on human chromosome 1.
• The protein contains a conserved bHLH domain that is predicted to mediate sequence-specific DNA binding and protein dimerization, characteristic of transcriptional regulators.
• NHLH1 is localized to the nucleus, consistent with its role in transcriptional regulation and cell fate determination.
• Functional studies suggest that NHLH1 may serve as a DNA-binding protein involved in the control of cell-type determination, possibly within the developing nervous system.
• It is annotated as a developmental protein and differentiation factor, indicating a role in early embryonic development and neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–60), which lies outside the conserved bHLH domain. Consider potential cross-reactivity with other bHLH family members if epitope similarity exists.
• For Western blotting, the predicted molecular weight is approximately 15 kDa. However, post-translational modifications, dimerization, or alternative translation start sites may result in bands at higher molecular weights; validate using appropriate controls.
• Due to its nuclear localization, this antibody may be suitable for chromatin immunoprecipitation (ChIP) assays. Optimize cross-linking and chromatin shearing conditions for your specific cell type or tissue.
• For immunoprecipitation, use non-denaturing lysis buffers to maintain protein–protein interactions, and include appropriate IgG controls to assess specificity.
• Cross-reactivity data is currently available only for human samples. If using in other species, verify sequence homology of the immunogen region and validate experimentally.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we are dedicated to supporting diagnostic manufacturers, research labs, and institutes with a reliable supply of IVD raw materials. This NHLH1 antibody is part of our extensive portfolio for neuroscience and transcription factor research. Beyond individual antibodies, we offer validated antibody pairs for ELISA, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets—helping you bridge the gap from concept to clinic efficiently.