Anti-NHLH1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q02575

Core Product Specifications and Parameters

Biological Background: NHLH1 Function and Localization

• NHLH1 (Helix-loop-helix protein 1) is a transcription factor encoded by the NHLH1 gene in humans, also designated as HEN1, NSCL, NSCL1, or bHLHa35.
• It belongs to the basic helix-loop-helix (bHLH) family, specifically the class A bHLH proteins, which are characterized by a conserved domain that mediates DNA binding and dimerization with other bHLH factors.
• NHLH1 functions as a DNA-binding protein that presumably regulates gene expression programs controlling cell-type determination, with a suggested role in the developing nervous system.
• Consistent with its transcriptional regulatory activity, NHLH1 localizes to the nucleus.
• Annotated keywords for NHLH1 include developmental protein, differentiation, DNA-binding, and transcription regulation, underscoring its participation in fundamental processes of development and gene control.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 60 amino acids of human NHLH1 (NP_005589.1); researchers may consider this region when designing experiments that require domain-specific detection.
• For Western blot (WB), anticipate a band near 15 kDa corresponding to the expected molecular weight; validate specificity with appropriate positive and negative control lysates from human sources.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect primarily nuclear staining; co-staining with a nuclear marker can confirm localization.
• For immunoprecipitation (IP), the antibody may capture NHLH1 and associated complexes; elution conditions should be optimized.
• In ELISA applications, assess the antibody for use as a capture or detection reagent against recombinant or endogenous NHLH1.
• Since cross-reactivity is indicated for human, perform initial testing in human-derived samples and verify reactivity if applied to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research laboratories, offering this NHLH1 rabbit monoclonal antibody alongside a wide portfolio of raw materials. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. From transcription factor research to developmental biology assay development, CamelBio helps streamline your procurement with a one-stop, B2B-focused approach.