Anti-NHEJ1 Polyclonal Antibody for WB and ELISA - Q9H9Q4

Core Product Specifications and Parameters

Biological Background: NHEJ1 Function and Localization

• NHEJ1, also known as XLF or Cernunnos, is a DNA repair protein essential for non-homologous end joining (NHEJ), double-strand break repair, and V(D)J recombination. Related references: PMID:16439204, PMID:16439205, PMID:17317666
• It promotes the ligation of various mismatched and non-cohesive DNA ends, facilitating efficient repair. Related references: PMID:17470781, PMID:17717001, PMID:19056826
• Together with PAXX, NHEJ1 collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. Related references: PMID:25670504, PMID:30250067
• NHEJ1 interacts with XRCC4 to form filaments or bridges that hold broken DNA ends in close proximity, stimulating ligation by the XRCC4-DNA ligase IV complex. Related references: PMID:21768349, PMID:21775435, PMID:22228831, PMID:27437582, PMID:28500754
• It binds DNA in a length-dependent manner, with preference for longer DNA substrates. Related references: PMID:17317666, PMID:18158905
• NHEJ1 is localized to the nucleus and chromosomes, consistent with its role in genome maintenance.
• It is ubiquitously expressed across human tissues, highlighting its fundamental role in DNA repair.
• Post-translational modifications include phosphorylation, and the protein is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen comprises full-length human NHEJ1 (amino acids 1-299), suggesting the antibody is suitable for detecting denatured NHEJ1 in Western blot (WB).
• For ELISA applications, consider coating with the recombinant immunogen or NHEJ1 peptides and titrate the antibody to determine optimal working concentrations.
• Validate the antibody’s specificity using NHEJ1 knockout cell lines or siRNA-mediated knockdown to confirm band disappearance in WB.
• Cross-reactivity with mouse NHEJ1 is reported; however, we recommend verifying reactivity in your specific mouse strain or tissue due to potential sequence variations.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to re-validate each new lot for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support NHEJ1 and DNA repair research. Our supply chain can also source rare-target raw materials, ensuring a seamless procurement experience from concept to clinic. Let CamelBio help you accelerate diagnostic development with reliable, high-quality reagents.