Anti-NgR3 (RTN4RL1) Rabbit Monoclonal Antibody for WB, IHC, IF - Q86UN2

Core Product Specifications and Parameters

Biological Background: NgR3 (RTN4RL1) Function and Localization

• Reticulon-4 receptor-like 1 (RTN4RL1), also known as Nogo receptor-like 2, Nogo-66 receptor homolog 2, or NgR3, is a GPI-anchored cell surface receptor predominantly expressed in the brain.
• It plays a functionally redundant role in postnatal brain development and regulating axon regeneration in the adult central nervous system; contributes to normal axon migration across the brain midline and formation of the corpus callosum.
• NgR3 inhibits neurite outgrowth and axon regeneration by binding to neuronal chondroitin sulfate proteoglycans and heparin, and mediates protection of motoneurons against apoptosis, likely through MAG interaction.
• It restricts the number of dendritic spines and synapses formed during brain development, signaling through Rho activation and downstream actin cytoskeleton reorganization. Related references: PMID:22325200
• Subcellular localization includes the cell membrane, membrane rafts, perikaryon, and cell projections, consistent with its receptor function in neurons.
• Besides the brain, lower expression levels are detected in kidney, lung, mammary gland, placenta, salivary gland, skeletal muscle, and spleen.
• Key structural features include a signal peptide, leucine-rich repeats, and a GPI-anchor, classifying it as a glycoprotein and lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 342-441 of human NgR3. This C-terminal region may ensure recognition of the full-length protein; however, validate specificity in your model system.
• For Western blot (WB), a band at approximately 49 kDa is expected. Use lysates from human, mouse, or rat brain tissue as positive controls.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), consider antigen retrieval and suitable permeabilization/fixation protocols optimized for neuronal tissues.
• For ELISA applications, the antibody's reactivity may be assessed against recombinant NgR3 protein or peptide; cross-reactivity with related family members should be evaluated.
• Cross-reactivity has been reported with mouse and rat, but users should confirm in the relevant sample context using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement. This anti-NgR3 antibody supports research in neuroscience and axon regeneration, and CamelBio can facilitate the acquisition of complementary reagents and technical consultation for your diagnostic development needs.