Anti-NGLY1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96IV0

Core Product Specifications and Parameters

Biological Background: NGLY1 Function and Localization

• NGLY1 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase), also known as N-glycanase 1 or PNGase, is a cytoplasmic enzyme responsible for deglycosylating misfolded N-linked glycoproteins.
• It specifically cleaves the β-aspartyl-glucosamine (GlcNAc) linkage, converting asparagine to aspartic acid, and displays a preference for high-mannose over complex-type oligosaccharides.
• NGLY1 plays a critical role in endoplasmic reticulum-associated degradation (ERAD) by recognizing misfolded glycoproteins exported from the ER to the cytosol, making them substrates for proteasomal degradation.
• The enzyme is a zinc metalloprotein, with metal binding essential for its hydrolase activity.
• NGLY1 is subject to post-translational modifications including acetylation and phosphorylation, and undergoes alternative splicing.
• Subcellularly, NGLY1 localizes to the cytoplasm, consistent with its function in cytosolic protein quality control.
• Mutations in NGLY1 are associated with NGLY1 deficiency, a congenital disorder of deglycosylation (CDDG), underscoring its importance in maintaining cellular proteostasis.

Experimental Guidance and Technical Tips

• The immunogen used spans the N-terminal region (aa 1-300) of human NGLY1, which may aid in detecting full-length and potentially truncated isoforms.
• For Western blot (WB), the predicted molecular weight is 74 kDa; consider using fresh samples with protease inhibitors to prevent degradation.
• This antibody has been validated by the supplier for WB, IHC-P, IF/ICC, and ELISA; each application may require individual optimization of sample preparation and antibody dilution.
• Cross-reactivity with mouse and rat is reported; verify reactivity in your experimental system, as epitope conservation may vary across tissues.
• As NGLY1 functions in the cytoplasm, subcellular fractionation or specific lysis buffers may improve detection in immunofluorescence (IF/ICC).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. This anti-NGLY1 monoclonal antibody supports investigations into glycoprotein quality control and ERAD pathways. As your sourcing partner, we can supply validated antibody pairs, bulk reagents, and assist in procuring rare-target raw materials to accelerate your assay development and research programs.