Anti-NGFRAP1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q00994

Core Product Specifications and Parameters

Biological Background: Protein BEX3 Function and Localization

• Protein identity: BEX3 is a brain-expressed X-linked protein that functions as a signaling adapter in nerve growth factor receptor (NGFR/p75NTR)-mediated apoptosis induced by NGF.
• Zinc-binding and apoptosis: BEX3 binds zinc and plays a role in zinc-triggered neuronal death.
• Pseudosubstrate activity: In the absence of reductive stress, BEX3 acts as a pseudosubstrate for the CRL2(FEM1B) complex by associating with FEM1B via zinc, preventing FEM1B from binding its normal substrates.
• Subcellular localization: BEX3 localizes to both the nucleus and cytoplasm, including the cytosol.
• Tissue distribution: Found in ovarian granulosa cells, testis, prostate, seminal vesicle, and liver.
• Post-translational modifications: BEX3 is subject to ubiquitin-like conjugation and contains zinc-binding sites.
• Alternative splicing: Multiple isoforms are produced by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-111 amino acids of human NGFRAP1, covering the full-length protein (expected molecular weight ~13 kDa). This recombinant fusion protein may generate antibodies recognizing multiple epitopes.
• For Western blot (WB), consider using reduced sample preparation and validate with positive control lysates from human or mouse tissues known to express BEX3 (e.g., ovary, testis, liver).
• For IF/ICC, note that BEX3 localizes to both nucleus and cytoplasm; consistent staining patterns should be observed.
• For ELISA, titrate the antibody and use appropriate blocking to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on BEX3-mediated apoptosis and neuronal death, this NGFRAP1 Rabbit pAb provides reliable detection across multiple applications. Our global supply chain can also source rare-target raw materials to support your assay development from concept to clinic.