Anti-NGF Polyclonal Antibody for WB, ELISA - P01138

Core Product Specifications and Parameters

Biological Background: NGF Function and Localization

• NGF, encoded by the NGF (NGFB) gene, is a secreted growth factor essential for the survival and maintenance of sympathetic and sensory neurons. Related references: PMID:14976160, PMID:20978020
• Through binding to NTRK1 and NGFR receptors, NGF initiates intracellular signaling cascades that promote neuronal growth, differentiation, and survival. Related references: PMID:20978020
• The unprocessed proNGF precursor binds the SORCS2/NGFR complex, triggering actin cytoskeleton reorganization, growth cone collapse, and pro-apoptotic signals (in vitro).
• NGF also inhibits metalloproteinase-mediated shedding of platelet glycoprotein VI, highlighting a role outside the nervous system. Related references: PMID:20164177
• The protein localizes primarily to the secretory pathway and endosome lumen; it undergoes cleavage at paired basic residues to generate the mature active form.
• Mutations in NGF are associated with hereditary sensory and autonomic neuropathy type 5 (HSAN5) and other neurodegenerative disorders.

Experimental Guidance and Technical Tips

• The immunogen spans residues 19–241 of human NGF (precursor), which includes the mature NGF sequence after proteolytic processing; the antibody may detect both proNGF and mature NGF forms.
• For Western blot, a band around 27 kDa is expected in reducing conditions; consider using brain or neuronal tissue lysates as positive controls.
• The confirmed cross-reactivity with mouse and rat NGF makes this antibody suitable for translational studies in common rodent models.
• For ELISA, preliminary checkerboard titrations are recommended to determine optimal coating antigen and antibody concentrations.
• Always validate the antibody's performance in your specific sample type and experimental context.

CamelBio: Your One-Stop Sourcing Bridge

For researchers focusing on neurotrophin signaling, CamelBio simplifies your procurement by providing a full spectrum of IVD raw materials—including validated anti-NGF antibodies, recombinant proteins, and ELISA-ready detection pairs. With reliable bulk supply and rare-target sourcing capabilities, we help you scale assays from bench to clinic while maintaining consistency.