Anti-NGDN Rabbit Polyclonal Antibody for WB, ELISA - Q8NEJ9

Core Product Specifications and Parameters

Biological Background: Neuroguidin Function and Localization

• NGDN (Neuroguidin), also designated CANu1, LCP5, or C14orf120, is an EIF4E-binding protein encoded by the NGDN gene in humans.
• It is an integral component of the small subunit (SSU) processome, the primary precursor of the small ribosomal subunit. Within the nucleolus, NGDN participates in pre-rRNA processing, facilitating RNA folding, modifications, rearrangements, and cleavage. Its timed dissociation governs the transition from the pre-A1 to pre-A1* assembly state. Related references: PMID:34516797
• NGDN functions as a translational repressor by inhibiting mRNA translation in a cytoplasmic polyadenylation element (CPE)-dependent manner.
• Subcellularly, the protein localizes to the nucleus, nucleolus, and centromere, as well as the cytoplasm and cell projections including axons, dendrites, and filopodia.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing generates multiple isoforms.
• The protein features coiled-coil domains and is involved in key processes such as ribosome biogenesis and translation regulation.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 61–209 of human NGDN; the resulting polyclonal antibody may detect multiple isoforms or post-translationally modified forms.
• For Western blotting, the predicted molecular weight is 36 kDa; additional bands arising from modifications or cleavage products should be considered and validated in relevant Human, Mouse, or Rat lysates.
• In ELISA applications, use the recombinant immunogen as a positive control and optimize coating conditions.
• As a polyclonal reagent, each batch may exhibit slight variations; it is advisable to include appropriate positive and negative controls and verify performance in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying validated polyclonal antibodies such as this NGDN Rabbit pAb for research and diagnostic development. From bulk ancillary reagents and optimized antibody pairs to customized monoclonal antibodies and rare-target raw materials, our integrated supply chain and technical expertise help streamline your project—from concept to clinical application—with a focus on ribosome biogenesis and translation regulation targets.