Anti-NG2/CSPG4 Rabbit Polyclonal Antibody [KO Validated] for WB, IHC-P, IF/ICC, ELISA - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2/CSPG4 Function and Localization

• NC2/CSPG4 is a large transmembrane proteoglycan that modulates cell proliferation, migration, and adhesion, playing critical roles in tissue remodeling, angiogenesis, and axon regeneration inhibition.
• It functions as a co-receptor for extracellular matrix components such as collagen type VI and growth factors, regulating matrix degradation through MMP16 and plasminogen activation.
• The intracellular domain interacts with scaffolding and signaling proteins, activating Rho GTPase, FAK, and ERK1/2 pathways to promote cell polarization and integrin-mediated adhesion.
• Subcellularly, NG2/CSPG4 localizes to the cell membrane, apical membrane, and lamellipodium, consistent with its role in cell motility.
• Tissue expression is prominent in fibroblasts, placenta, and malignant melanoma cells, with protein-level detection in normal and tumor contexts. Related references: PMID:36213313 PMID:32337544
• Keywords from UniProt highlight its involvement in angiogenesis, differentiation, and tissue remodeling, with documented disulfide bond formation, glycosylation, and phosphorylation.

Experimental Guidance and Technical Tips

• For western blot, consider using a low-percentage SDS-PAGE gel and extended transfer time due to the high molecular weight of NG2/CSPG4 (~251 kDa).
• The polyclonal nature may yield robust signal in immunohistochemistry; be sure to validate the antibody in relevant cell lines or tissues known to express NG2/CSPG4.
• In immunofluorescence, permeabilization may be unnecessary if targeting the extracellular region (immunogen aa 1340-1563); however, experimental conditions should be optimized for each cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, encompassing optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For studies on melanoma, angiogenesis, and matrix biology, we offer comprehensive support from antibody pairs to technical consultation, helping accelerate your projects from concept to clinic.