Anti-NG2/CSPG4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2/CSPG4 Function and Localization

• NG2/CSPG4 is a chondroitin sulfate proteoglycan, also known as melanoma chondroitin sulfate proteoglycan (MCSP), encoded by the CSPG4 gene. It is a large transmembrane protein with an extracellular domain harboring chondroitin sulfate glycosaminoglycan chains.
• Functions as a cell surface receptor for collagen alpha 2(VI) and various growth factors, modulating cell proliferation, migration, and microvascular morphogenesis. It also inhibits neurite outgrowth and growth cone collapse during axon regeneration.
• Plays a critical role in melanoma cell invasion by regulating MPP16-dependent degradation of type I collagen and enhancing plasminogen activation while inhibiting angiostatin, thereby promoting tissue remodeling and angiogenesis.
• Acts as a signal transducer via its cytoplasmic C-terminus, interacting with scaffolding and signaling proteins to promote retraction fiber formation, cell polarization, and integrin-mediated adhesion through Rho GTPase, CDC42, ACK1, and BCAR1 pathways.
• Activates FAK and ERK1/ERK2 signaling cascades, contributing to cell spreading and migration.
• Localizes primarily to the cell membrane, including the apical cell membrane, lamellipodium membrane, and cell surface projections.
• Detected at the protein level in fibroblasts, placenta, and malignant melanoma cells, underscoring its importance in cancer progression and developmental processes.
• Post-translational modifications include phosphorylation, disulfide bond formation, and N-linked glycosylation. It features a transmembrane helix and a signal peptide, classifying it as a type I transmembrane proteoglycan.

Experimental Guidance and Technical Tips

• The immunogen (aa 1950–2210) resides in the cytoplasmic domain; therefore, the antibody may detect both full-length and truncated forms of CSPG4 in western blotting.
• Due to the large molecular weight (~251 kDa), consider using a low-percentage SDS-PAGE gel (e.g., 6%) and extended transfer times for optimal WB results.
• For IHC-P, heat-induced antigen retrieval may be required to expose the epitope in formalin-fixed, paraffin-embedded tissues.
• In IF/ICC, permeabilization steps are essential to allow antibody access to the intracellular epitope.
• The confirmed cross-reactivity with mouse enables comparative studies, but validation in the specific sample system is recommended due to potential species-dependent differences.
• When designing ELISA assays, pairing this antibody with a capture antibody targeting an extracellular epitope may yield effective sandwich immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement. For CSPG4/NG2-related investigations in cancer, angiogenesis, and developmental biology, our team can help identify the ideal reagent configurations to accelerate your IVD development.