Anti-NG2/CSPG4 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, FC, ELISA - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2/CSPG4 Function and Localization

• NG2/CSPG4 (Chondroitin sulfate proteoglycan 4) is a large transmembrane proteoglycan, also known as melanoma chondroitin sulfate proteoglycan (MCSP), that belongs to the NG2 family and plays critical roles in cell adhesion, migration, and signaling.
• It functions as a cell surface receptor for collagen alpha 2(VI), modulating cell migration on collagen substrates, and interacts with growth factors and extracellular matrix proteases to regulate their activity.
• NG2/CSPG4 stimulates endothelial cell motility during microvascular morphogenesis, contributing to angiogenesis, and may inhibit neurite outgrowth and growth cone collapse during axon regeneration.
• The protein participates in tissue remodeling by modulating the plasminogen system, enhancing plasminogen activation and inhibiting angiostatin, and may regulate MPP16-dependent degradation of type I collagen, facilitating melanoma cell invasion.
• Through its cytoplasmic C-terminus, NG2/CSPG4 acts as a signal transducer by binding scaffolding and signaling proteins, activating Rho GTPases to promote retraction fiber formation and cell polarization, and stimulating integrin-mediated adhesion via CDC42, ACK1, and BCAR1, leading to FAK and ERK1/ERK2 signaling cascade activation.
• Subcellularly, NG2/CSPG4 localizes to the cell membrane, apical cell membrane, and lamellipodium membrane, consistent with its roles in cell motility and membrane dynamics.
• Tissue specificity: detected at protein level in fibroblasts and placenta, with prominent expression in malignant melanoma cells, linking it to developmental and cancer biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region towards the C-terminus of NG2/CSPG4 (aa 1583-2224); consider validating in the relevant species and sample types for each application.
• For Western blot (WB), the predicted molecular weight is ~251 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), test fixation and antigen retrieval conditions to optimize signal, particularly in tissues where NG2 is expressed (e.g., placenta, fibroblasts, melanoma).
• For flow cytometry (FC), use appropriate cell lines known to express NG2 (e.g., melanoma cell lines) for validation.
• For ELISA, the antibody may be used as capture or detection antibody; pairing with other clones or formats may be necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including this anti-NG2/CSPG4 antibody for cancer biology and angiogenesis research. With a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents, we support your development from concept to clinic. Our dedicated sourcing services can also secure rare-target raw materials to meet customized project needs.