Anti-NG2 Monoclonal Antibody for Flow Cytometry - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2 (CSPG4) Function and Localization

• NG2, also known as chondroitin sulfate proteoglycan 4 (CSPG4) or melanoma-associated chondroitin sulfate proteoglycan (MCSP), is a large cell-surface proteoglycan implicated in cell proliferation, migration, and angiogenesis.
• It acts as a receptor for collagen alpha-2(VI), facilitating cell migration on collagen-rich substrates, and binds growth factors and extracellular proteases via its N-terminal domain, modulating their activity.
• NG2 inhibits neurite outgrowth and growth cone collapse, suggesting a role in restricting axon regeneration after injury.
• The cytoplasmic C-terminus functions as a signal transduction hub, interacting with scaffolding and signaling proteins to promote retraction fiber formation, cell polarization through Rho GTPase activation, and integrin-mediated adhesion via the CDC42-ACK1-BCAR1 cascade.
• NG2 enhances FAK and ERK1/ERK2 signaling, further supporting its role in cell motility and invasion, particularly in melanoma cells where it may aid type I collagen degradation.
• It is detected in fibroblasts and placenta at the protein level, and is highly expressed in malignant melanoma cells. Related references: PMID:36213313 PMID:32337544
• Subcellular localization includes the cell membrane, apical membrane, lamellipodium membrane, and cell surface, consistent with its roles in adhesion and migration.
• Post-translational modifications include extensive glycosylation, phosphorylation, and disulfide bond formation, contributing to its structural integrity and interactions.

Experimental Guidance and Technical Tips

• When using this conjugate for flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and assay conditions.
• Include appropriate isotype controls and viability dyes in your panel to ensure accurate gating and identification of NG2-positive populations.
• Validate antibody performance on known NG2-expressing cell lines, such as human melanoma cells or primary fibroblasts, to confirm staining patterns.
• Because the immunogen spans the C-terminal region (aa 1583-2224), the antibody should recognize the intact full-length proteoglycan; however, target validation in your sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials for oncology and cell surface marker research, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. As your one-stop sourcing bridge, we support diagnostic manufacturers and laboratories in accelerating assay development for targets like NG2 (CSPG4), covering every stage from concept to clinic.