Anti-NFYC Rabbit Polyclonal Antibody for WB and ELISA - Q13952

Core Product Specifications and Parameters

Biological Background: NFYC Function and Localization

• NFYC (also known as NF-YC, H1TF2A, HAP5, CBFC, CBF-C) is a subunit of the sequence-specific heterotrimeric transcription factor NF-Y.
• NF-Y specifically binds to the 5'-CCAAT-3' box motif present in the promoters of various target genes, regulating their transcription.
• Depending on its interacting cofactors, NF-Y can function as either a transcriptional activator or repressor, providing regulatory flexibility.
• The protein is localized to the nucleus, consistent with its role in DNA-binding and transcriptional regulation.
• NFYC undergoes alternative splicing, producing isoforms that may have distinct functional properties.
• The predicted molecular weight of NFYC is approximately 50 kDa (UniProt: 50302 Da), consistent with the reported protein weight of 50 kDa for this antibody.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 27-120) of human NFYC; this region is well conserved, so the antibody may detect multiple isoforms.
• For Western blot, a band around 50 kDa is expected; validate the antibody in your specific cell or tissue lysate and consider using a positive control.
• The antibody shows cross-reactivity with rat, making it suitable for translational studies in rodent models.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve an optimal signal-to-noise ratio.
• As a polyclonal antibody, performance may vary between batches; always titrate and validate for each new application or lot.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers streamlined access to high-quality antibodies like this NFYC polyclonal antibody, supporting transcription factor research and gene regulation studies. Our one-stop sourcing capabilities cover validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and even rare target raw-material procurement—empowering diagnostic manufacturers and research labs to accelerate development from concept to clinical application.