Anti-NFYB Rabbit Polyclonal Antibody for WB, ELISA - P25208

Core Product Specifications and Parameters

Biological Background: NFYB Function and Localization

• NFYB encodes the beta subunit of the nuclear transcription factor Y (NF-Y), a heterotrimeric complex that includes NF-YA and NF-YC subunits. This complex specifically binds to the CCAAT box motif (5'-CCAAT-3') in eukaryotic promoters.
• NF-Y can act as a transcriptional activator or repressor, depending on its interacting cofactors, allowing it to fine-tune gene expression in response to various signaling pathways.
• Consistent with its transcription factor role, NFYB localizes exclusively to the nucleus, where it participates in DNA binding and transcriptional regulation.
• The protein is subject to post-translational modifications including ubiquitination and isopeptide bond formation, which may modulate its stability, interactions, or activity.
• NFYB is also known by aliases HAP3, CBF-A, CBF-B, and NF-YB, reflecting its historical identification in different experimental contexts.
• With a calculated molecular weight of approximately 22.8 kDa, the full-length human NFYB protein comprises 207 amino acids, as covered by the immunogen used to generate this antibody.

Experimental Guidance and Technical Tips

• For Western blotting, starting antibody dilutions should be empirically optimized; typical rabbit polyclonal antibodies may work in the range of 1:500–1:2000, but validation in the relevant tissue or cell lysate is essential.
• The antibody is reported to detect human and mouse NFYB. When working with other species, perform sequence alignment and consider testing cross-reactivity experimentally.
• In ELISA applications, the antibody can be employed as a capture or detection reagent; pairing with an appropriate secondary antibody is required for signal generation.
• As NFYB is a nuclear protein, using nuclear extraction protocols is recommended to enrich the target and minimize background from cytoplasmic proteins.
• Since the immunogen spans the nearly full-length protein (aa 1–207), the polyclonal antibody likely recognizes multiple epitopes, but be aware of potential isoform-specific reactivity if splice variants exist.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For transcription regulation and DNA-binding protein research, CamelBio facilitates sourcing of hard-to-find targets like NFYB, ensuring you can advance from assay concept to clinical application efficiently. Our dedicated support bridges your procurement needs with reliable supply chains for critical raw materials.