Anti-NFU1 Polyclonal Antibody for WB and ELISA - Q9UMS0

Core Product Specifications and Parameters

Biological Background: NFU1 Function and Localization

• NFU1 (also known as HIRA-interacting protein 5) is a mitochondrial iron-sulfur cluster scaffold protein that belongs to the Nfu family.
• It functions in the assembly of [4Fe-4S] clusters and delivers them to target proteins, a process essential for cellular iron homeostasis and mitochondrial energy metabolism.
• Localizes primarily to mitochondria and the cytosol, reflecting its role in both organellar and cytoplasmic iron-sulfur cluster biogenesis.
• Ubiquitously expressed across tissues, with relatively weak expression in adult lung compared to fetal lung.
• Contains a transit peptide for mitochondrial import and is associated with iron-sulfur and metal-binding activities.
• Genetic variants in NFU1 are linked to disorders such as hereditary spastic paraplegia and primary mitochondrial disease, highlighting its importance in neurodegeneration.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates or mitochondrial fractions derived from human, mouse, or rat cell lines; the predicted molecular weight is ~28 kDa.
• Validate antibody specificity using appropriate controls such as siRNA knockdown or overexpression of NFU1.
• For ELISA applications, optimize coating concentrations and detection conditions based on your assay format and sample matrix.
• The immunogen corresponds to the N-terminal region (amino acids 1-230) of human NFU1, which may influence epitope recognition; verify reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials. This anti-NFU1 polyclonal antibody supports investigations into iron-sulfur cluster biology and mitochondrial dysfunction. Contact us for complementary antibody pairs, bulk reagent orders, and tailored sourcing solutions for rare targets.