Anti-NFS1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y697

Core Product Specifications and Parameters

Biological Background: NFS1 Function and Localization

• NFS1 (also known as IscS, NIFS, or COXPD52) is a pyridoxal phosphate-dependent cysteine desulfurase that releases sulfur from L-cysteine to L-alanine for iron-sulfur (Fe-S) cluster biogenesis. Related references: PMID:18650437 PMID:29097656 PMID:31101807
• NFS1 is the catalytic subunit of the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1), which generates persulfide sulfur that is transferred to the scaffold protein ISCU, a step assisted by frataxin (FXN).
• The core ISC assembly complex, including NFS1, is critical for the de novo synthesis of [2Fe-2S] clusters, the first step in mitochondrial iron-sulfur protein biogenesis, with subsequent cluster transfer to chaperone proteins HSCB, HSPA9, and GLRX5.
• NFS1 also acts as a sulfur donor for MOCS3, contributing to molybdenum cofactor biosynthesis. Related references: PMID:16527810 PMID:18650437 PMID:23593335
• NFS1 localizes primarily to mitochondria but has been detected in the cytoplasm, nucleus, and centrosome.
• Predominantly expressed in heart and skeletal muscle, with lower levels in brain, liver, and pancreas.
• Mutations in NFS1 are associated with primary mitochondrial disease, and the enzyme requires pyridoxal phosphate as a cofactor.
• Alternative initiation and splicing generate multiple isoforms of NFS1.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 290–457, C-terminal) may detect full-length NFS1 and certain isoforms; consider verifying in the relevant sample system.
• For western blotting, the predicted molecular weight is ~50 kDa, but post-translational modifications or alternative splicing may result in additional bands.
• In IHC-P and IF/ICC, optimize antigen retrieval and fixation conditions, and validate using positive and negative tissue controls.
• For ELISA applications, this polyclonal antibody can be used for capture or detection after appropriate pairing optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a reliable one-stop supply of IVD raw materials, including this high-quality anti-NFS1 polyclonal antibody tailored for mitochondrial iron-sulfur cluster biogenesis studies. Beyond antibodies, our portfolio spans validated antibody pairs, recombinant antigens, and extensive ancillary reagents, all backed by rigorous quality control to accelerate your assay development from concept to clinic. For niche or rare target sourcing, our dedicated technical consulting bridges the gap between your project's needs and clinical-ready solutions.