Anti-NFRKB Rabbit Monoclonal Antibody for WB, ELISA - Q6P4R8

Core Product Specifications and Parameters

Biological Background: NFRKB Function and Localization

• NFRKB (Nuclear factor related to kappa-B-binding protein), also known as INO80 complex subunit G (INO80G), is a DNA-binding protein that recognizes the consensus sequence 5'-GGGGAATCTCC-3'.
• It is a regulatory component of the chromatin remodeling INO80 complex, which plays essential roles in transcriptional regulation, DNA replication, and DNA repair.
• NFRKB modulates the deubiquitinase activity of UCHL5 within the INO80 complex, thereby influencing chromatin dynamics.
• It is predominantly localized to the nucleus, consistent with its role in chromatin-associated processes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• NFRKB is expressed in multiple tissues, including thymus, brain, testes, spleen, and liver.
• The protein is subject to alternative splicing, potentially generating distinct isoforms with tissue-specific functions.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein encompassing amino acids 370-495 of human NFRKB (NP_001137307.1). For western blot (WB), consider testing in human cell lines or tissues known to express NFRKB, such as thymus or spleen.
• In ELISA applications, use appropriate coating conditions and detection systems; validation with purified protein or cell lysates is recommended.
• As cross-reactivity is reported only for human, verify performance if applied to other species.
• The predicted molecular weight is 139 kDa; confirm band size with appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For targets like NFRKB—a key component of the chromatin remodeling INO80 complex—we offer validated monoclonal antibodies, optimized antibody pairs, and comprehensive ancillary reagents to support your assay development from concept to clinic. Contact us for bulk orders and custom sourcing solutions.