Anti-NFKB1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor which is processed into the active p50 subunit. p105 functions as a cytoplasmic retention factor for NF-kappa-B proteins, while p50 constitutes the active NF-kappa-B complex with RELA/p65. Related references: PMID:1423592

• NF-kappa-B is a pleiotropic transcription factor involved in inflammation, immunity, cell growth, tumorigenesis, and apoptosis. It exists as homo- or heterodimers of Rel-like domain-containing proteins, with the p65-p50 heterodimer being the most abundant form.

• The NF-kappa-B p65-p50 complex binds specifically to the kappa-B consensus sequence 5'-GGRNNYYCC-3' in enhancer regions of target genes, regulating immune and acute-phase responses. Related references: PMID:1740106, PMID:7830764

• NFKB1 is localized to both the cytoplasm and nucleus, with inactive NF-kappa-B complexes retained in the cytoplasm by I-kappa-B inhibitors. Upon stimulation, I-kappa-B is phosphorylated and degraded, allowing nuclear translocation of NF-kappa-B.

• Post-translational modifications such as phosphorylation, acetylation, and ubiquitination regulate NF-kappa-B activity.

• The p50-p50 homodimer acts as a transcriptional repressor but can become an activator when associated with BCL3.

• NFKB1 also plays a role in MAPK signaling repression by complexing with MAP3K8; proteasome-dependent degradation of p105 releases active MAP3K8.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 740-964 of NFKB1, which is located in the C-terminal region of p105. Therefore, the antibody is expected to detect the full-length p105 precursor (~105 kDa) rather than the processed p50 subunit. Consider this when designing experiments to distinguish p105 from p50.
• For Western blot, a band at approximately 105 kDa is anticipated. Optimize loading controls and antibody dilution for your sample system.
• In immunoprecipitation, validate the antibody’s ability to capture native NFKB1 complexes under non-denaturing conditions.
• For IHC-P and IF/ICC, confirm subcellular localization (cytoplasmic vs. nuclear) under basal and stimulated conditions to reflect NF-kappa-B activation status.
• ELISA applications may require titration of the antibody against recombinant antigen or cell lysates to establish a suitable working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive supply of raw materials for NF-kappa-B pathway analysis, including this anti-NFKB1 polyclonal antibody. Our portfolio includes validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate assay development for inflammation and immunity research. We specialize in sourcing rare-target raw materials, ensuring a reliable supply chain from concept to clinic.