Anti-NFIL3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16649

Core Product Specifications and Parameters

Biological Background: NFIL3 Function and Localization

• NFIL3 (Nuclear factor interleukin-3-regulated protein), also known as E4BP4, IL3BP1, NFIL3A, or NF-IL3A, is a transcriptional regulator encoded by the NFIL3 gene in humans (GeneID: 4783). It recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', present in many cellular and viral promoters.
• Acts as both a transcriptional repressor and activator. It represses transcription from promoters with ATF sites and competes with PAR DNA-binding factors (DBP, HLF, TEF) for the same consensus site. It activates transcription from the interleukin-3 promoter in T-cells.
• NFIL3 is a core component of the circadian clock, functioning as a negative regulator of PER2 expression, thereby influencing circadian oscillation.
• Subcellularly localized to the nucleus, consistent with its role as a transcription factor. It is expressed in various tissues including bladder, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow, and muscle.
• Post-translationally modified by phosphorylation and ubiquitination (isopeptide bond, Ubl conjugation), which may regulate its activity and stability.
• Plays a role in immune cell development; it protects pro-B cells from apoptosis and is required for natural killer cell precursor development.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 133-462 of human NFIL3, covering a large portion of the protein. This region includes the DNA-binding domain and likely critical functional motifs, making the antibody suitable for detecting full-length NFIL3.
• For Western blot (WB), the predicted molecular weight is 51 kDa. Use appropriate positive controls (e.g., lysates from tissues with high NFIL3 expression such as lymph node or bone marrow) and verify band specificity using knockdown or knockout samples if available.
• For immunofluorescence/immunocytochemistry (IF/ICC), ensure proper fixation and permeabilization to access nuclear NFIL3. Include a nuclear counterstain to confirm localization.
• For ELISA, consider pairing with a secondary antibody optimized for rabbit polyclonal detection. Validate assay conditions using recombinant NFIL3 protein or cell lysates.
• Since the antibody cross-reacts with rat NFIL3, it can be used for cross-species studies, but always confirm reactivity in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, accelerating the development of diagnostic assays from concept to clinic. For researchers and manufacturers focused on circadian rhythms, transcriptional regulation, or immune cell development, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise includes sourcing rare-target raw materials like anti-NFIL3 antibodies, ensuring robust supply for your critical research and diagnostic applications.