Anti-NFIC Rabbit Polyclonal Antibody for WB, ELISA - P08651

Core Product Specifications and Parameters

Biological Background: NFIC Function and Localization

• NFIC (Nuclear factor 1 C-type), also known as CTF (CCAAT-box-binding transcription factor), NFI-C, or TGGCA-binding protein, is a member of the Nuclear factor I (NFI) family of transcription factors.
• It specifically recognizes and binds the palindromic DNA sequence 5'-TTGGCNNNNNGCCAA-3', present in many viral and cellular promoters and in the adenovirus type 2 origin of replication.
• NFIC functions as both a transcriptional activator and a replication factor, regulating gene expression and DNA synthesis.
• Recent evidence indicates that NFIC promotes transcription of the telomerase reverse transcriptase (TERT) gene, linking it to telomere maintenance. Related references: PMID:35198878
• The protein is localized in the nucleus, consistent with its role in DNA binding and transcriptional control.
• Post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond formation), modulate NFIC activity and stability.
• Alternative splicing produces multiple NFIC isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using whole-cell or nuclear extracts from human, mouse, or rat cell lines. The expected molecular weight of NFIC is approximately 56 kDa; validate antibody concentration empirically for your sample system.
• For ELISA applications, employ standard indirect ELISA protocols using recombinant NFIC protein or peptide fragments as coating antigens. Optimize coating concentration and blocking conditions.
• Given that the immunogen covers the N-terminal region (amino acids 1-220), the antibody may detect multiple NFIC isoforms; confirm specificity in the relevant species by knockdown or overexpression controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-NFIC rabbit pAb. Our one-stop platform also supplies bulk ancillary reagents and supports rare target raw-material sourcing to accelerate your assay development from concept to clinic.