Anti-NFIB/NF1B2 Rabbit Monoclonal Antibody for WB and ELISA - O00712

Core Product Specifications and Parameters

Biological Background: NFIB Function and Localization

• Nuclear factor 1 B-type (NFIB) is also known as CCAAT-box-binding transcription factor (CTF), Nuclear factor I/B (NF-I/B, NFI-B), and TGGCA-binding protein.
• Acts as a transcriptional activator of GFAP, playing an essential role in proper brain development. Related references: PMID:30388402
• Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2.
• Capable of individually activating transcription and replication, indicating a direct role in both processes.
• Localizes to the nucleus, consistent with its function as a DNA-binding transcription factor.
• Subject to alternative splicing, producing multiple isoforms that may vary in transcriptional activity.
• Post-translationally modified by phosphorylation and methylation, which may regulate its activity and interactions.
• Associated with disease variants linked to intellectual disability, highlighting its clinical relevance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 321–420, a region that may be conserved across isoforms or species; consider validating reactivity in human and mouse systems.
• For Western blotting, whole-cell lysates from neural tissues or cell lines expressing NFIB (such as brain-derived cells) are recommended; a band at approximately 47 kDa is expected.
• In ELISA applications, the antibody can be used for capturing or detection when paired with a suitable antigen (e.g., recombinant NFIB protein or peptide).
• As a rabbit monoclonal antibody, it offers consistent lot-to-lot reproducibility; nevertheless, optimization of dilution factors and incubation conditions is advised based on the specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers with a wide portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This anti-NFIB monoclonal antibody is tailored for studies in transcriptional regulation and neurodevelopmental research, fitting seamlessly into workflows that require robust detection of NFIB. Beyond single reagents, CamelBio offers bulk supply, customized antibody pairs, and sourcing for rare targets to accelerate your projects from concept to clinical application.