Anti-NFIA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12857

Core Product Specifications and Parameters

Biological Background: NFIA Function and Localization

• NFIA (Nuclear factor 1 A-type) is a member of the nuclear factor I (NFI) family of transcription factors, also known as CCAAT-box-binding transcription factor (CTF), NF-I/A, and TGGCA-binding protein.
• It recognizes and binds the palindromic consensus sequence 5'-TTGGCNNNNNGCCAA-3', present in viral and cellular promoters and in the adenovirus type 2 origin of replication.
• NFIA functions as both a transcriptional activator and a regulator of DNA replication, individually capable of initiating transcription and replication from appropriate templates.
• Subcellular localization is predominantly nuclear (UniProt: SUBCELLULAR LOCATION: Nucleus).
• Post-translational modifications include phosphorylation and methylation, which may regulate its activity and interactions.
• Multiple protein isoforms are generated through alternative splicing, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-330 of human NFIA, a central region conserved across isoforms; thus the antibody may detect multiple splice variants.
• In Western blot (WB), a major band around 56 kDa is expected based on the predicted molecular weight; validate with appropriate positive control lysates (e.g., HeLa, HEK293, or tissue extracts from human, mouse, or rat).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval methods and optimize antibody dilution in the target tissue or cell line.
• As a polyclonal antibody, background may vary between batches; include appropriate negative controls and consider pre-adsorption if non-specific binding occurs.
• This antibody has been reported to work in ELISA; for quantitative applications, couple with a matched detection antibody and validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering a comprehensive portfolio from validated antibodies to ancillary reagents. This anti-NFIA rabbit polyclonal antibody exemplifies our commitment to providing high-quality tools for transcription and DNA replication research. Beyond individual reagents, we supply optimized antibody pairs, bulk monoclonal and polyclonal antibodies, and can assist in sourcing rare-target raw materials—streamlining your development from concept to clinic.