Anti-NFE2L3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y4A8

Core Product Specifications and Parameters

Biological Background: NFE2L3 Function and Localization

• NFE2L3 (Nuclear Factor, Erythroid 2 Like 3), also known as NRF3, is a member of the cap'n'collar (CNC) family of transcription factors.
• It functions as a transcriptional activator, specifically activating erythroid-specific globin gene expression, indicating a role in erythropoiesis.
• The protein localizes predominantly to the nucleus, consistent with its DNA-binding and transcription regulatory activities.
• NFE2L3 is highly expressed in human placenta and in B-cell and monocyte cell lines, with low expression observed in heart, brain, lung, skeletal muscle, kidney, and pancreas.
• It contains domains critical for DNA binding and transcription activation, contributing to its regulatory functions.
• The full-length protein has a calculated molecular weight of approximately 76 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 330–550 of human NFE2L3, covering the C-terminal region; consider that epitopes recognized by this polyclonal antibody may include conserved domains.
• For Western blot analysis, use nuclear extracts or whole-cell lysates from cell lines with known high NFE2L3 expression, such as placental or B-cell lines, to maximize detection.
• In immunohistochemistry (paraffin), antigen retrieval methods may be optimized; human placenta serves as a suitable positive control tissue due to its high expression.
• Due to the protein's nuclear localization, signal in IHC-P should be expected primarily in the nucleus; confirm specificity with appropriate negative controls.
• Cross-reactivity with mouse and rat orthologs is reported; validate expected band sizes (around 76 kDa) in relevant species-specific samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable, one-stop channel for IVD raw-material sourcing. This anti-NFE2L3 polyclonal antibody is ideal for studies on transcriptional regulation in erythropoiesis, and we can support your projects with optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets. Let us bridge your development needs from concept to commercialization.