Anti-NFE2L1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14494

Core Product Specifications and Parameters

Biological Background: NFE2L1 Function and Localization

• NFE2L1 (NRF1/TCF11/LCR-F1) is a CNC-type bZIP transcription factor that functions as an endoplasmic reticulum membrane sensor. It translocates to the nucleus upon cleavage by DDI2 in response to stresses such as cholesterol excess, oxidative stress, or proteasome inhibition. Related references: PMID:20932482, PMID:24448410
• In the nucleus, NFE2L1 heterodimerizes with small Maf proteins (MAFF, MAFG, MAFK) and binds antioxidant response elements (AREs), thereby regulating genes critical for oxidative stress defense and detoxification. Related references: PMID:8932385, PMID:9421508
• It acts as a key sensor of cholesterol excess: at the ER membrane, it directly binds cholesterol via its CRAC motif, blocking its cleavage and activation, thus allowing expression of cholesterol removal genes such as CD36.
• NFE2L1 is essential for proteasome homeostasis; upon proteasome inhibition, it activates a transcriptional program that upregulates proteasome subunit genes to restore proteasome function. Related references: PMID:20932482
• During fetal liver hematopoiesis, NFE2L1 plays a protective role against oxidative damage and contributes to hepatic lipid homeostasis.
• Together with CEBPB, NFE2L1 represses DSPP expression during odontoblast differentiation, and in osteoblasts, it activates SP7/Osterix in response to ascorbic acid. Related references: PMID:15308669
• Subcellularly, NFE2L1 is anchored to the endoplasmic reticulum membrane via a signal-anchor transmembrane helix; upon stress signals, it is proteolytically released and translocates to the nucleus to exert its transcriptional functions.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 515–772) corresponds to a region in the C-terminal half of human NFE2L1. This region includes domains potentially involved in DNA binding and dimerization. When used in WB, a band around 85 kDa is expected for the full-length protein; shorter isoforms or cleavage products may also be observed.
• For IHC-P, consider antigen retrieval protocols (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0). Optimize antibody concentration based on tissue type and fixation.
• In IF/ICC, ensure appropriate permeabilization to detect nuclear and ER-localized protein. Co-staining with organelle markers may aid interpretation.
• For ELISA, the antibody can be used as capture or detection reagent, but pair validation is recommended.
• Cross-reactivity with mouse and rat NFE2L1 has been reported; however, confirm performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner for diagnostic manufacturers and research laboratories, CamelBio provides high-quality raw materials including this NFE2L1 rabbit polyclonal antibody. We support oxidative stress, cholesterol metabolism, and proteostasis research areas with validated antibodies, bulk ancillary reagents, and customized sourcing services. Whether you need optimized antibody pairs, recombinant proteins, or rare target raw materials, CamelBio streamlines supply from concept to clinic.