Anti-NFATC3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12968

Core Product Specifications and Parameters

Biological Background: NFATC3 Function and Localization

• NFATC3 (Nuclear factor of activated T-cells, cytoplasmic 3), also known as NFAT4 or NFATx, is a member of the NFAT family of transcription factors that regulate gene expression in response to calcium signaling.
• Binds to DNA promoter regions to control transcription of target genes, including TNFSF11/RANKL in osteoblasts and IL-2 in T-cells. Related references: PMID:18815128
• Translocates from the cytoplasm to the nucleus upon calcium influx via ORAI1 CRAC channels, enabling transcriptional activation of downstream targets.
• Plays a role in cardiac development and cardiomyocyte survival; forms a complex with STAT3 and NFATC4 to activate CRYAB transcription.
• Promotes smooth muscle hypertrophy and differentiation by inducing ACTA2 expression in response to EDN1 and ROCK signaling.
• Predominantly expressed in thymus and skeletal muscle, with weaker expression in kidney, heart, testis, and other tissues. Related references: PMID:9759864
• Subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which modulate its activity and subcellular localization.
• Also functions in macrophage-mediated innate immunity by promoting NOS2 transcription and nitric oxide production for pathogen clearance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–290) of human NFATC3, which may detect multiple isoforms; consider validating isoform specificity in your experimental system.
• For Western blot (WB), a band near 116 kDa is expected. Validate detection using positive control tissues (e.g., thymus, skeletal muscle) and appropriate loading controls.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), include antigen retrieval and proper negative controls to confirm staining specificity.
• This antibody is compatible with ELISA; determine optimal working dilutions through titration experiments.
• Cross-reactivity with mouse and rat has been documented; verify reactivity if using samples from other species.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and research labs with high-quality antibodies, antigens, and ancillary reagents. This NFATC3 antibody aids studies in transcriptional regulation, immune signaling, and cardiac biology. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and custom sourcing for rare targets—helping you move seamlessly from concept to clinic.