Anti-NFATC3 Monoclonal Antibody for WB, IF-P, ELISA - Q12968

Core Product Specifications and Parameters

Biological Background: NFATC3 Function and Localization

• NFATC3 is a member of the nuclear factor of activated T-cells (NFAT) family of transcription factors, also known as NFAT4 or NFATx. It plays a role in Ca(2+)-mediated transcriptional responses upon Ca(2+) influx via ORAI1 CRAC channels.
• Acts as a transcriptional activator by binding to the promoter regions of target genes, including TNFSF11/RANKL and IL-2, thereby promoting their expression. Related references: PMID:18815128
• In T-cells, NFATC3 is critical for the inducible expression of cytokine genes, especially IL-2, contributing to immune response regulation.
• Predominantly expressed in thymus and skeletal muscle, with weaker expression in peripheral blood leukocytes, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta, and pancreas. Related references: PMID:9759864
• Subcellular localization includes both cytoplasm and nucleus; it shuttles to the nucleus upon activation to regulate gene transcription.
• Subject to post-translational modifications such as acetylation, phosphorylation, and ubiquitination, which modulate its activity and protein interactions.
• Involved in embryonic heart development by maintaining cardiomyocyte survival, proliferation, and differentiation, in conjunction with NFATC4.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 850-1075) of human NFATC3; confirm reactivity in your specific model system.
• For Western blot, a band around 116 kDa is expected; use appropriate molecular weight markers and loading controls to verify specificity.
• In immunofluorescence on paraffin-embedded sections (IF-P), antigen retrieval may be required; optimize fixation and permeabilization conditions for your sample.
• For ELISA, consider using the antibody as a capture or detection reagent after thorough validation for your assay format.
• The antibody cross-reacts with mouse and rat NFATC3; validate specificity in these species using knockout or knockdown controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to provide a comprehensive range of IVD raw materials. For transcription factor and immunology studies, we supply validated antibodies like this NFATC3 rabbit mAb, along with optimized antibody pairs, bulk ancillary reagents, and support for rare target sourcing. From concept to clinic, CamelBio helps streamline your assay development with reliable, one-stop access to critical reagents.