Anti-NFAT5 Polyclonal Antibody for WB, ELISA - O94916

Core Product Specifications and Parameters

Biological Background: NFAT5 Function and Localization

• NFAT5, also known as T-cell transcription factor NFAT5, tonicity-responsive enhancer-binding protein (TonEBP), NFATZ, OREBP, NF-AT5, NFATL1, and TONEBP, is a member of the nuclear factor of activated T-cells family.
• Functions as a transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes, mediating the cellular response to hypertonicity. Related references: PMID:10377394 PMID:11485737 PMID:41652173 PMID:10051678
• Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' to regulate target gene expression. Related references: PMID:10377394 PMID:11485737
• Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Related references: PMID:22266867
• Involved in the DNA damage response by preventing formation of R-loops, which are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. Related references: PMID:34049076
• Predominantly localizes to the nucleus, but can also be found in the cytoplasm and associated with chromosomes.
• Widely expressed, with highest levels in skeletal muscle, brain, heart, and peripheral blood leukocytes.
• Post-translationally modified by acetylation, phosphorylation, ADP-ribosylation, and ubiquitination, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1355-1455) of human NFAT5; this sequence may be conserved across isoforms, making the antibody suitable for detecting total NFAT5 protein in Western blot.
• For Western blot, consider using a low-percentage gel and extended transfer times due to the large molecular weight (~166 kDa) of NFAT5.
• The antibody reactivity is validated for human NFAT5 by WB and ELISA; cross-reactivity with other species or family members should be tested experimentally.
• In ELISA applications, the antibody can be considered as a capture or detection reagent after appropriate validation in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, supporting projects from concept to clinic. This anti-NFAT5 antibody is especially relevant for studies on osmotic stress signaling, inflammatory gene regulation, and DNA damage repair pathways. Contact CamelBio for customized sourcing solutions, including rare target raw materials and technical consulting to accelerate your IVD development.