Anti-NFAT2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95644

Core Product Specifications and Parameters

Biological Background: NFAT2 Function and Localization

• NFAT2 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATc, NF-ATC, or NF-ATc1.2, is encoded by the NFATC1 gene and belongs to the NFAT transcription factor family.
• It plays a key role in the inducible expression of cytokine genes in T-cells, particularly IL-2 and IL-4, and controls T-cell activation, proliferation, differentiation, and programmed death. Related references: PMID:10358178
• NFAT2 is required for osteoclastogenesis and regulates numerous genes critical for osteoclast differentiation and function.
• The protein localizes to both the cytoplasm and nucleus, translocating to the nucleus upon activation signals to regulate gene transcription.
• Isoforms exhibit differential tissue expression: preferential expression in effector T-cells (isoforms A) versus naive T-cells (isoforms B and C), with additional variant-specific patterns in muscle, brain, placenta, and thymus.
• Subject to phosphorylation and alternative splicing, NFAT2 contains DNA-binding domains, repeat regions, and can function as either a transcriptional activator or repressor.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–300 of the N-terminal region; consider its suitability for detecting total NFAT2 protein or specific isoforms in relevant sample systems.
• As a polyclonal antibody, it may recognize multiple isoforms and processed forms; validation by appropriate controls (e.g., knockout samples or multiple detection methods) is advised.
• For applications such as WB, IHC-P, IF/ICC, and ELISA, optimized protocols and antibody dilutions should be empirically determined using positive (e.g., T-cell lysates) and negative controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target materials. For researchers investigating T-cell biology, cytokine signaling, or bone metabolism, we streamline procurement from concept to clinic with reliable supply solutions.