Anti-NFAT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95644

Core Product Specifications and Parameters

Biological Background: NFAT2 Function and Localization

• NFAT2 (Nuclear factor of activated T-cells, cytoplasmic 1) is a transcription factor belonging to the NFAT family; alternative names include NF-ATc and NFATc. Multiple isoforms are generated by alternative initiation and splicing.
• Core function: Inducible expression of cytokine genes in T-cells, particularly IL-2 and IL-4, and gene regulation in embryonic cardiac cells (PubMed:10358178). Related references: PMID:10358178
• Regulates activation, proliferation, differentiation, and programmed death of T-lymphocytes and other lymphoid/non-lymphoid cells (PubMed:10358178). Related references: PMID:10358178
• Required for osteoclastogenesis and osteoclast differentiation and function (By similarity).
• Subcellular localization: Shuttles between cytoplasm and nucleus; dephosphorylation triggers nuclear translocation.
• Tissue specificity: Expressed in thymus, peripheral leukocytes (T-cells), and spleen. Isoforms A are preferentially in effector T-cells, isoforms B/C in naïve T-cells.
• Keywords highlight its role as a DNA-binding phosphoprotein with activator/repressor functions in transcription regulation, supported by structural studies (3D-structure).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 115-298 of NFAT2 (isoform IA, NP_001265598.1), a region conserved across several isoforms; consider verifying reactivity with the specific isoform(s) of interest.
• For WB, predicted molecular weight is ~101 kDa; use nuclear/cytoplasmic extracts from human T-cell lines (e.g., Jurkat) to detect endogenous NFAT2, and validate with appropriate controls.
• In IHC-P, test on human lymphoid tissues (tonsil, thymus) and optimize antigen retrieval conditions; nuclear or cytoplasmic staining may be observed depending on activation state.
• ELISA: Use the antibody with recombinant NFAT2 protein; perform titration to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical support for diagnostic development. For NFAT2 targets in T-cell biology and immunology research, we provide validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets to streamline your assay development pipeline.