Anti-NF-κB2 Monoclonal Antibody for WB, IHC-P, ELISA - Q00653

Core Product Specifications and Parameters

Biological Background: NF-κB2 Function and Localization

• NF-κB2 encodes the p100 subunit, which undergoes proteolytic processing to generate the active DNA-binding p52 subunit; isoform p49 is also produced by alternative splicing.
• p100/p52 belongs to the NF-kappa-B transcription factor family, forming homo- or heterodimers with Rel-like proteins (RELA, RELB, REL, NFKB1) to regulate gene expression.
• In the non-canonical NF-kappa-B pathway, p100 associated with RelB is phosphorylated by IKKα and processed to p52, leading to nuclear translocation of the RelB-p52 transcriptional activator complex.
• p52 homodimers act as transcriptional repressors, while RelB-p52 heterodimers activate transcription, binding to kappa-B sites (5'-GGRNNYYCC-3') in promoters of immune and inflammatory genes.
• NF-κB2 is involved in a wide range of biological processes including inflammation, immunity, cell growth, differentiation, and apoptosis.
• In concert with RELB, NF-κB2 regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.
• Subcellular localization is both cytoplasmic (as inactive p100/I-kappa-B complexes) and nuclear (as active p52-containing dimers).
• Ank repeats, isopeptide bond, and ubl conjugation (SUMOylation) are post-translational modifications, and the protein is also phosphorylated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 745-877 within the C-terminal ankyrin repeat domain of p100, which is absent in the processed p52 subunit; therefore, the antibody may preferentially recognize full-length p100 (~97 kDa). Consider validating reactivity against both p100 and p52 in your model system if required.
• For western blotting, use standard SDS-PAGE and transfer conditions; a band near 97 kDa is expected for p100. Lower bands may represent processing intermediates or degradation products.
• In immunohistochemistry (IHC-P), both nuclear and cytoplasmic staining may be observed depending on the activation status of NF-κB2 in the tissue. Include appropriate controls for signal specificity.
• For ELISA, the antibody may be used with recombinant p100 or cell lysates; ensure the coating antigen contains the C-terminal epitope region.
• Cross-reactivity with mouse and rat suggests conservation of the immunogenic sequence among these species, but sequence alignment is recommended to confirm epitope identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing, technical services, and consulting—from concept to clinic. For NF-κB2 and other NF-kappa-B pathway targets, suppliers on CamelBio can provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with rare target raw-material procurement.